| Abstract: | Growth of C. roseus cell suspension culture was defined in termsof dry weight, cell number, mitotic index, and packed cell volume.Removal of major nutrients from the medium was monitored asa function of culture growth. Phosphate and sucrose were theonly macronutrients completely exhausted. Utilization of thesetwo nutrients occurred parallel with increments in dry weightand cell number. Increasing the nutrient medium levels of sucroseand phosphate prolonged growth of this culture; lag and exponentialphases were extended; cell number and dry weight yield weredoubled. Dry weight assimilation was enhanced by increasingthe nutrient medium level of sucrose, whereas increments incell number were related to phosphate level. Two alkaloid fractions(fractions 1 and 2) were identified in this cell line. Fraction2 alkaloid level declined as the nutrient medium supply of nitrogenwas depleted. |
