D-L amino acid analysis using automated precolumn derivatization with 1-fluoro-2,4-dinitrophenyl-5-alanine amide |
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Authors: | A. Scaloni M. Simmaco F. Bossa |
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Affiliation: | (1) Dipartimento di Scienze Biochimiche A. Rossi Fanelli, Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Università La Sapienza, Piazzale Aldo Moro 5, I-00185 Roma, Italy |
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Abstract: | Summary An automated procedure for the precolumn derivatization of enantiomeric amino acid mixtures with 1-fluoro-2,4-dinitrophenyl-5-alanine amide and a liquid chromatographic method for the separation of the derivatives with UV detection are reported. The system described allows to perform routine analyses using microbore columns with a sensitivity at the picomol level. Improvements for the use of this reagent in the protocol of a subtractive Edman degradation procedure of peptides to determine the sequence position of amino acid residues with concomitant identification of their chirality are also described. |
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Keywords: | Amino acid-enantiomeric mixtures Marfey reagent Edman degradation RP-HPLC |
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