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Characterization of PsMPK2, the first C1 subgroup MAP kinase from pea (<Emphasis Type="Italic">Pisum sativum</Emphasis> L.)
Authors:Dolores Ortiz-Masia  Miguel A Perez-Amador  Pablo Carbonell  Fernando Aniento  Juan Carbonell  Maria J Marcote
Institution:(1) Facultad de Farmacia, Universidad de Valencia, Avda. Vicente Andrés Estellés s/n, 46100 Burjassot, Spain;(2) Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas y Universidad Politécnica de Valencia, Avda. de los Naranjos s/n, 46022 Valencia, Spain
Abstract:Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as in biotic and abiotic stress responses. They are classified according to their sequence homology into four major groups (A–D). A large amount of information about MAPKs in groups A and B is available but few data of the C group have been reported. In this study, a C1 subgroup MAP kinase cDNA, PsMPK2, was isolated from Pisum sativum. PsMPK2 is expressed in vegetative (root and leaf) and reproductive (stamen, pistil and fruit) organs. Expression of PsMPK2 in Arabidopsis thaliana shows that mechanical injury and other stress signals as abscisic acid, jasmonic acid and hydrogen peroxide increase its kinase activity, extending previous results indicating that C1 subgroup MAPKs may be involved in the response to stress.
Keywords:Abscisic acid  Anther            Arabidopsis            Hydrogen peroxide  Jasmonic acid  MAP kinase  Pea  Wounding
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