Ribosomal DNA as a convenient probe to follow segregation and possible divergency from expected homozygosity after haploidization of an androgenetic process |
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Authors: | A. Benslimane C. Hartmann J. de Buyse Y. Henry E. Picard A. Rode |
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Affiliation: | (1) Laboratoire de Biologie Moléculaire Végétale, Université Paris XI, Bâtiment 430, F-91405 Orsay, France;(2) Laboratoire d'Amélioration des Plantes, Université Paris XI, Bâtiment 360, F-91405 Orsay, France;(3) Groupement d'Intérêt Scientifique du Moulon, La Ferme du Moulon, F-91190 Gif-sur-Yvette, France |
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Abstract: | Summary Restriction fragment length polymorphism of the wheat nuclear ribosomal DNA has been studied in several steps of a breeding scheme, including parental genotypes, F1 hybrid, F9 generation, and anther-derived doubled haploid lines obtained from F9. Ribosomal DNA represents a suitable molecular marker in following segregation and possible divergency from expected homozygosity after haploidization of an androgenetic process. It has been shown to undergo variations among the first cycle-doubled haploid lines in the relative amount of two different sizes of ribosomal DNA repeat units. The specificity and peculiar properties of the plant system used allowed us to assign an intrachromosomal location (short arm of the chromosomes 1B, 1R or 6B) to several ribosomal DNA repeat units that differ by the length of their nontranscribed spacer region. |
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Keywords: | Ribosomal DNA Restriction fragment length polymorphism Anther-derived doubled haploids Gametoclonal variation Wheat |
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