Extractive fermentation in cloud point system for lipase production by Serratia marcescens ECU1010 |
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| Authors: | Tao Pan Zhilong Wang Jian-He Xu Zhenqiang Wu Hanshi Qi |
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| Institution: | (1) School of Bioscience and Bioengineering, South China University of Technology, Guangzhou, 510006, People’s Republic of China;(2) School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China;(3) Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China; |
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| Abstract: | Extractive microbial fermentation for production of lipase by Serratia marcescens ECU1010 has been carried out in cloud point system. The cloud point system is composed of mixture nonionic surfactants with
a ratio of Triton X-114 to Triton X-45 4:1 in aqueous solution. The lipase prefers to partition into the surfactant rich phase
(coacervate phase) whereas the cells and other hydrophilic proteins retain in the dilute phase of cloud point system. Thus,
a concentration factor 4.2-fold and a purification factor 1.3-fold of the lipase have been achieved in the extractive fermentation
process. This is the first report about extractive fermentation of proteins in cloud point system. |
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