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日本血吸虫成虫培养细胞的超微结构观察
引用本文:董惠芬,蒋明森,杨明义,李瑛,钟沁萍,周述龙. 日本血吸虫成虫培养细胞的超微结构观察[J]. 动物学报, 1999, 45(1): 1-7
作者姓名:董惠芬  蒋明森  杨明义  李瑛  钟沁萍  周述龙
作者单位:湖北医科大学寄生虫学教研室、血吸虫病研究室,武汉,430071
基金项目:湖北省武汉市青年科技晨光计划;CGB940104;
摘    要:报告日本血吸虫成虫培养细胞的超微结构。在透射电镜下日本血吸虫成虫培养细胞呈多角形、圆颗粒形,三角扇形和鞭毛形等多种形状,其中以多角形为主。细胞表面光滑,或有乳头乳突起,微绒毛和微饮泡等。胞质内有不同数量的线粒体,内质网,核糖体和糖原颗粒等分布,高尔基复合体很少或无;其中线粒体超微结构的变化可以作为评判培养条件优劣的一个指标。核常呈圆形,核膜为一单位膜,核孔清晰;核内具较丰富的异染色质,核膜内缘常有

关 键 词:日本血吸虫  成虫  培养细胞  超微结构

ULTRASTRUCTURE OF CULTURED CELLS FROM ADULT SCHISTOSOMA JAPONICUM
DONG Hui-Fen,JIANG Ming-Sen,YANG Ming-Yi,LI Ying,ZHONG Qin-Ping,ZHOU Shu-Long. ULTRASTRUCTURE OF CULTURED CELLS FROM ADULT SCHISTOSOMA JAPONICUM[J]. Acta Zoologica Sinica, 1999, 45(1): 1-7
Authors:DONG Hui-Fen  JIANG Ming-Sen  YANG Ming-Yi  LI Ying  ZHONG Qin-Ping  ZHOU Shu-Long
Abstract:Ultrastructure of cultural cells from adult Schistosoma japonicum was observed. Several types of cells including polygonal, round granular, deltaic fan shaped and flagellated cells were found, of which polygonal cells were dominant. There were papillae, microvilli or microvesicles on the surface of some cells. Less or more mitochondria, endoplasmic reticula, ribosomes and glycogen granules existed in the cytoplasm. However, Very few or no Golgi complexes were found. The ultrastructural changes of mitochondria could be used to estimate whether the culture conditions were appropriate or not. Nucleus was round, with round nucleolus inside and clear pores on the nuclear membrane. There was much heterochromatin in the nucleus, some of which located just inside the nuclear membrane was lumpish heterochromatin. However, Cells from different tissues had different structural characteristics. Germ cells were characterized by relatively high nucleus/cytoplasm ratios, many vacuoles in different sizes and several types of cortical granules. There were vitelline droplets and globules composed of very high electronic density granules in vitelline cells, cilium clusters in flame cells, and many round or elliptical vacuoles in nerve cells. Among the above four kinds of cells, vetelline cells were most and nerve cells were least in number.
Keywords:Schistosoma japonicum   Adult   Cultural cell   Ultrastructure
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