Protective effects of proline against cadmium toxicity in micropropagated hyperaccumulator, Solanum nigrum L. |
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Authors: | Jin Xu HengXia Yin Xia Li |
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Institution: | (1) Center for Agricultural Resources Research, Institute of Genetic and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, People’s Republic of China |
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Abstract: | Solanum nigrum is a newly discovered Cd-hyperaccumulator. In the present study, the protective effects of proline against cadmium toxicity
of callus and regenerated shoots of S. nigrum are investigated based on a high frequency in vitro shoot regeneration system. Proline pretreatment reduces the reactive
oxygen species levels and protects the plasma membrane integrity of callus under cadmium stress, and therefore improves the
cadmium tolerance in S. nigrum. Inductively coupled plasma mass spectroscopy analysis shows that exogenous proline increases the cadmium accumulation in
callus and regenerated shoots of S. nigrum. Further analysis indicates that the improvement of cadmium tolerance caused by proline pretreatment is correlated with an
increase of superoxide dismutase and catalase activity and intracellular total glutathione content. The interaction between
proline and enzymic or non-enzymic antioxidants is discussed. |
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Keywords: | In vitro culture Growth regulator Heavy metal tolerance Antioxidant systems |
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