Introduction of active enzymes into intact Escherichia coli cells by means of liposomes. Phenotypic suppression of uvr A and pol A mutants |
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| Authors: | S E Bresler L A Noskin O K Kaboev I M Stepanova E F Davidenkova O A Rosenberg E I Schwartz A G Beketova and A T Akhmedov |
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| Institution: | (1) Leningrad Institute of Nuclear Physics, Academy of Sciences of the USSR, Gatchina, Leningrad, USSR;(2) Present address: Research Division of Prof. E.F. Davidenkova, Leningrad, USSR |
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| Abstract: | Summary Genetically deficient cells were supplied with the missing enzymes, purified from an independent source. The introduction of exogenous enzymes into the cells was effected by two independent methods: plasmolysis and liposome transformation. The latter procedure yielded a homogenous cell population which had been rescued from the defect even if the molecular weight of the enzyme amounted to 70 KD (Kilodaltons). |
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