Benomyl: A broad spectrum fungicide for use in plant cell and protoplast culture |
| |
Authors: | Randal M. Hauptmann Jack M. Widholm Jack D. Paxton |
| |
Affiliation: | (1) Departments of Agronomy and Plant Pathology, University of Illinois, 1102 S. Goodwin Ave., 61801 Urbana, IL, USA |
| |
Abstract: | The systemic fungicide methyl-1-(butylcarbamoyl)-2-benzimidazole carbamate (benomyl), is a broad spectrum fungicide. Benomyl at concentrations up to 50 mg/l does not inhibit the growth of suspension cultures ofNicotiana tabacum, Datura innoxia, Daucus carota, Glycine canescens, andSolanum tuberosum nor growth ofN. tabacum orN. plumbaginifolia protoplasts if benomyl is dissolved by autoclaving or boiling. Addition of benomyl dissolved in dimethyl sulfoxide results in a visible toxicity. Benomyl, at 6.25–50 mg/l preventsPenicillium spp. growth in both protoplast and cell cultures and can be used to remove fungal contaminates after one to three transfers without visibly retarding plant cell growth. Due to the broad spectrum of fungicidal activity, and nontoxicity at high concentrations when dissolved by boiling or autoclaving, benomyl can be used effectively to control or prevent fungal contamination in plant cell and protoplast cultures. |
| |
Keywords: | Nicotiana tabacum L. cv. xanthi N. plumbaginifolia Viv. Glycine canescens F. J. Herm. Daucus carota L. var sativa Solanum tuberosum L. cv. Merrimac Datura innoxia Mill. benomyl fungicide plant cell culture protoplast culture |
本文献已被 SpringerLink 等数据库收录! |
|