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A role for SENSITIVE TO FREEZING2 in protecting chloroplasts against freeze-induced damage in Arabidopsis |
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| Authors: | Fourrier Nicolas Bédard Jocelyn Lopez-Juez Enrique Barbrook Adrian Bowyer John Jarvis Paul Warren Gareth Thorlby Glenn |
| Institution: | School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey TW20 0EX, UK,; Department of Biology, University of Leicester, University Road, Leicester LE1 7RH, UK, and; Department of Biochemistry, University of Cambridge, Hopkins Building, Downing Site, Cambridge CB2 1QW, UK |
| Abstract: | The SENSITIVE TO FREEZING2 ( SFR2 ) gene has an important role in freezing tolerance in Arabidopsis thaliana . We show that homologous genes are present, and expressed, in a wide range of terrestrial plants, including species not able to tolerate freezing. Expression constructs derived from the cDNAs of a number of different plant species, including examples not tolerant to freezing, are able to complement the freezing sensitivity of the Arabidopsis sfr2 mutant. In Arabidopsis the SFR2 protein is localized to the chloroplast outer envelope membrane, as revealed by the analysis of transgenic plants expressing SFR2 fusions to GFP, by confocal microscopy, and by the immunological analysis of isolated chloroplasts treated with thermolysin protease. Moreover, the chloroplasts of the sfr2 mutant show clear evidence of rapid damage after a freezing episode, suggesting a role for SFR2 in the protection of the chloroplast. |
| Keywords: | Freezing tolerance SFR2 Arabidopsis chloroplast |
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