| 大叶醉鱼草内生真菌LL3026菌株杀虫活性及ITS-5.8S rDNA序列分析 |
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| 引用本文： | 郝蕾,陈钧,胥峰.大叶醉鱼草内生真菌LL3026菌株杀虫活性及ITS-5.8S rDNA序列分析[J].天然产物研究与开发,2011,23(2):286-290. |
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| 作者姓名： | 郝蕾 陈钧 胥峰 |
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| 作者单位： | 1. 江苏大学药学院
2. 江苏大学生命科学研究院,镇江,212013 |
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| 摘 要： | 从大叶醉鱼草的叶子中分离得到一株内生真菌LL3026,以卤虫模型测稀释后发酵液的杀虫活性,结果表明LL3026发酵液杀虫活性较强,且温度、光照及紫外照射对LL3026发酵液杀虫活性影响不显著;采用分子生物学方法对LL3026菌株rDNA的ITS基因(ITS-5.8S rDNA)进行PCR扩增、测序,构建系统发育树。ITS基因显示其属于刺盘孢属真菌。
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| 关 键 词： | 醉鱼草 内生真菌 杀虫活性 稳定性 5.8Sr DNA D1/D2 序列分析 |
Insecticidal Activity and Sequences Analysis of ITS-5.8S rDNA of Endophyfic Fungi LL3026 from Buddleja davidii |
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| HAO Lei,CHEN Jun,XU Feng.Insecticidal Activity and Sequences Analysis of ITS-5.8S rDNA of Endophyfic Fungi LL3026 from Buddleja davidii[J].Natural Product Research and Development,2011,23(2):286-290. |
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| Authors: | HAO Lei CHEN Jun XU Feng |
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| Institution: | HAO Lei1,CHEN Jun1,XU Feng21College of Pharmacy,Jiangsu University,2Institute of Life Science,Zhenjiang 212013,China |
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| Abstract: | An endophytic fungus LL3026 was isolated from leaves of Buddleja davidii.The insecticidal activity of the diluted fermentation broth was tested through the effect of anti-brine shrimp,and the results showed that the insecticidal activity was very strong.Revised mortality was remained 100% till LL3026 fermentation broth was diluted to 1/16,and LD50 was 55.There were no significant changes when temperature and the time of irradiation and UV were varied.The complete 5.8S rDNA sequence of LL3026 was amplified a... |
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| Keywords: | 5 8S rDNA D1/D2 |
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