Use of sorbose to enhance cellobiase activity in a Trichoderma reesei cellulase system produced on wheat hydrolysate

Summary Several methods were investigated for increasing cellobiase activity of T. reesei cellulases produced using an acid-hydrolyzed wheat flour. The use of sorbose in cellulase production is preferred to mixed cultures with Aspergillus niger. Using 13.5 g/L wheat hydrolysate and 1.0 g/L sorbose, the culture of T. reesei produced 3.72 IU/mL of filter paper activity (FPA) and 0.53 IU/mL of cellobiase activity. The addition of sorbose resulted in 89% increase in cellobiase activity and 47% increase in FPA.