Micropropagation and induction of autotetraploid plants of <Emphasis Type="Italic">Chrysanthemum cinerariifolium</Emphasis> (Trev.) Vis |
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Authors: | Zhen Liu Shanlin Gao |
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Institution: | (1) School of Bioengineering, Southwest Jiaotong University, Chengdu, Sichuan Province, People’s Republic of China, 610031;(2) Department of Genetics and Breeding, China Pharmaceutical University, P.O. Box D-02, 1 Shennong Road, Ji Xiang An, Nanjing, Jiangsu Province, People’s Republic of China, 210038 |
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Abstract: | Rapid propagation technology was established and optimized in vitro for Chrysanthemum cinerariifolium (Trev.) Vis., an important botanical insecticide plant with a huge international market. A large number of buds could be
induced directly from epicotyl and hypocotyl explants on Murashige T; Skoog F. J. Plant. Physiol. 15: 473–479; (1962) medium
Murashige and Skoog (MS) medium] supplemented with 0.3 mg l−1 benzyladenine (BA) and 0.3 mg l−1 α-naphthaleneacetic acid (NAA). Root induction and development could be observed within 15 d after inoculation on 1/2 MS
medium supplemented with 0.2 mg l−1 indole-3-acetic acid (IAA) and 0.1 mg l−1 rooting powder (ABT). Furthermore, a polyploid breeding study in vitro was reported to obtain superior breeding lines with high yield and good quality. Autotetraploid lines of C. cinerariifolium were obtained by colchicine treatments and identified by root-tip chromosome determination and stoma observation. The chromosome
number of the autotetraploid plantlet was 2N = 4x = 36. Obtained autotetraploid lines will be of important genetic and breeding value and be used for further selection and
plant breeding. |
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Keywords: | Chromosome determination Chrysanthemum cinerariifolium Micropropagation Polyploidy Pyrethrum |
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