Three-dimensional structure of living chloroplasts as visualized by confocal scanning laser microscopy |
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Authors: | E. A. van Spronsen V. Sarafis G. J. Brakenhoff H. T. M. van der Voort N. Nanninga |
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Affiliation: | (1) Department of Molecular Cell Biology, Section of Molecular Cytology, University of Amsterdam, The Netherlands;(2) Hawkesbury Agricultural College, Richmond, New South Wales |
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Abstract: | Summary The newly developed confocal scanning laser microscope, together with image processing by computer, has been used to obtain three-dimensional information on the organization of grana in chloroplasts in living plant tissue. Chloroplasts are ideally suited for such studies because their pigments show bright autofluorescence. The high-resolution stereo images bridge a gap between classic light microscopy and electron microscopy. Our preliminary observations on several plant species resemble most the early observations of Strugger (1951: Die Strukturordnung im Chloroplasten. Ber Deutsch Bot Ges 64: 69–83) and suggest that the 3-D technique might well be suitable to solve discrepancies in the interpretation of classical light microscopic and electron microscopic observations.Abbreviations 3-D three dimensional - CSLM confocal scanning laser microscopy - DCMU 3-(3,4-dichlorophenyl)-1,1-dimethyl urea - DNA deoxyribonucleic acid |
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Keywords: | Chloroplasts Confocal scanning laser microscopy Image processing Three-dimensional modelling |
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