Molecular typing of HLA-B27 alleles |
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Authors: | O. Dominguez E. Coto E. Martinez-Naves S. Y. Choo C. López-Larrea |
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Affiliation: | (1) Inmunologîa, Hospital Central, 33006 Oviedo, Spain;(2) Fred Hutchinson Cancer Research Center, Seattle, WA, USA;(3) Servicio de Inmunologia, Hospital Central, 33006 Oviedo, Spain |
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Abstract: | HLA-B27 represents a family of closely related antigens. Six alleles which differ in a limited number of nucleotide substitutions have been described (B*2701—B*2706). These changes are clustered in 1 and 2 domains. Polymerase chain reaction strategies were designed to amplify specific regions of class I exons 2 and 3. Amplified sequences were tested with eight sequence-specific oligonucleotides to distinguish all B27 subtypes. We also subtyped B27 in 50 healthy Spanish individuals using this procedure. The B*2705 subtype is over-represented in our population (96%). The remaining 4% carried the B*2702 allele. This finding is in agreement with the frequencies described by other techniques (cytotoxic T lymphocytes and isoeletric-focusing) for Caucasian populations. Class I oligotyping is a poorly developed field with significant potential applications. This procedure of genotyping B27 alleles is a reliable method which can be used in transplantation and B27-associated disease studies. |
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