Proliferation of epithelial cells derived from rat dorsolateral prostate in serum-free primary cell culture and their response to androgen |
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Authors: | Nozomu Nishi Yuhsi Matuo Takahisa Nakamoto Fumio Wada |
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Institution: | (1) Department of Endocrinology, Kagawa Medical School, 1750 Miki-cho, Kita-gun, 761-07 Kagawa, Japan;(2) Present address: Department of Urology, School of Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734 Hiroshima, Japan |
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Abstract: | Summary Primary cultured epithelial cells derived from the rat dorsolateral prostate proliferated in serum-free nutrient medium WAJC
404 supplemented with mitogens: insulin (650 nM), cholera toxin (120 pM), epidermal growth factor (EGF) (2.5 nM), dexamethasone (300 nM), and bovine pituitary extract (25 μg/ml). The culture consisted of two types of epithelial cell colonies: one originated
from single cells or small cell aggregates and the other was epithelial cell outgrowth from small tissue fragments attached
to a substratum. There were differences in requirements for the mitogens between the two types of colonies. Requirements for
cholera toxin, bovine pituitary extract, and dexamethasone were higher in the former type of colonies, and those for EGF were
higher in the latter type of colonies. Proliferation of the epithelial cells in either type, of colony was suppressed more
than 50% by 1 nM dihydrotestosterone. This suppressive effect was not mediated by stromal component in the tissue fragments, and was counteracted
by cyproterone acetate, indicating specific and direct action of the androgen on prostate epithelial cells. The results suggest
that there is discrete participation of polypeptide growth factors and androgen in proliferation and differentiation, respectively,
of prostate epithelial cells in vivo. |
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Keywords: | dorsolateral prostate epithelial cells primary culture serum-free media androgen growth factors |
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