Beneficial effects of enhanced aeration using perfluorodecalin
in <Emphasis Type="Italic">Yarrowia lipolytica</Emphasis> cultures for lipase production |
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Authors: | Priscilla F Fonseca Amaral Ana Paula R de Almeida Tathiana Peixoto Maria Helena M Rocha-Leão João A P Coutinho Maria Alice Z Coelho |
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Institution: | (1) Departamento de Eng. Bioquímica, Escola de Química, Universidade Federal do Rio de Janeiro, 21949-900 R.J., Brazil;(2) CICECO, Departamento de Química, Universidade de Aveiro, 3810-193 Aveiro, Portugal |
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Abstract: | The inadequate supply of oxygen to biomass is a critical factor to the productivity of most aerobic submerged fermentations.
This happens because oxygen is sparingly soluble in the aqueous media. The use of a second liquid phase of perfluorocarbon
(PFC), an oxygen-carrying compound, in the culture medium can increase the availability of oxygen to the microorganisms. The
effect of perfluorodecalin on Yarrowia lipolytica cultures was investigated in shake-flask cultures. It was found that the specific growth rate of Y. lipolytica, a strictly aerobic yeast, increases with increasing PFC concentration. Extracellular lipase production was increased with
20% (v/v) of PFC and agitation of 250 rev/min. It was shown that the PFC presence benefitted lipase production and not just
its secretion to the extracellular medium. |
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Keywords: | Lipase Oxygen Perfluorocarbon Yarrowia lipolytica |
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