Monochloramine suppresses the proliferation of colorectal cancer cell line Caco‐2 by both apoptosis and G2/M cell cycle arrest |
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Authors: | Tetsuya Kohda Satoru Sakuma Muneyuki Abe Yohko Fujimoto |
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Affiliation: | Laboratory of Physiological Chemistry, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka, Japan |
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Abstract: | The aim of this study was to assess a possible role of monochloramine (NH2Cl), one of the reactive chlorine species, which induce oxidative stress, on the proliferation of colorectal cancer cell line Caco‐2. At concentrations ranging from 10 to 200 μM, NH2Cl (14–61% inhibition), but not hypochlorous acid, dose‐dependently inhibited the cell viability of Caco‐2 cells. Experiments utilizing methionine (a scavenger of NH2Cl), taurine‐chloramine and glutamine‐chloramine revealed that only NH2Cl affects the cancer cell proliferation among reactive chlorine species, with a relative specificity. Furthermore, flow‐cytometry experiments showed that the anti‐proliferative effect of NH2Cl is partially attributable to both apoptosis and G2/M cell cycle arrest. These results suggest that NH2Cl has the potential to suppress colorectal cancer cell proliferation. Copyright © 2013 John Wiley & Sons, Ltd. |
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Keywords: | monochloramine reactive chlorine species colon cancer proliferation oxidative stress |
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