Isolation and characterization of plant growth-promoting strain Pantoea NII-186. From Western Ghat Forest soil, India |
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Authors: | S.G. Dastager C.K. Deepa S.C. Puneet C.S. Nautiyal A. Pandey |
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Affiliation: | Biotechnology Division, National Institute for Interdisciplinary Science and Technology (CSIR), Trivandrum, India; National Botanical Research Institute, Rana Pratap Marg, Lucknow, India |
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Abstract: | Aims: To isolate plant growth-promoting bacterium from Western Ghat forests in India. Methods and Results: A Gram-negative, rod shaped, cream white coloured strain Pantoea NII-186 isolated from Western Ghat soil sample. The taxonomic position of the bacterium was confirmed by sequencing of 16S rRNA and phylogenetic analysis. A strain grew at a wide range of temperature ranging from 5–40°C, but optimum growth was observed at 28–30°C. It showed multiple plant growth-promoting attributes such as phosphate solubilization activity, indole acetic acid (IAA) production, siderophore production and HCN production. It was able to solubilize (28 μg of Ca3PO4 ml−1 day−1), and produce IAA (59 μg) at 28°C. The solubilization of insoluble phosphate was associates with a drop in the pH of the culture medium. Pantoea sp. NII-186 tolerate to different environmental stresses like 5–40°C, 0–7% salt concentration and 4–12 pH range. Conclusions: The 16S rRNA gene sequence confirmed that the isolate NII-186 was belongs to Pantoea genus and showed considerable differences in physiological properties with previously reported species of this genus. Isolate NII-186 possessed multiple attributes of plant growth-promoting activity. Significance and Impact of the Study: Hence in the context it is proposed that Pantoea sp. NII-186, could be deployed as an inoculant to attain the desired plant growth-promoting activity in agricultural environment. |
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Keywords: | Pantoea species plant growth-promoting rhizobacteria activity Western Ghat forest soils |
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