口服维生素D对实验性脑疟小鼠树突状细胞的影响

维生素D（VD）为固醇类衍生物，除发挥抗佝偻病作用，还具有一定的免疫调节功能。主要研究VD对实验性脑疟小鼠树突状细胞的影响。结果显示，与对照组相比，在伯氏疟原虫（P.bANKA）感染前给予VD，可降低C57BL/6小鼠发生脑型疟疾的风险，存活时间明显延长。VD预处理后，脾脏中髓样树突状细胞（CD11c⁺CD11b⁺）和浆样树突状细胞（CD11c⁺B220⁺）百分率明显降低，CD11c⁺MHCII⁺细胞、CD11c⁺TLR4⁺细胞和CD11c⁺TLR9⁺细胞的百分含量也显著减少。由此提示，预防性口服VD可抑制感染小鼠树突状细胞的数量和功能，对脑型疟疾的发生具有一定预防作用，为新型抗疟药物的研发提供参考。

Effects of Pre Treatment with Vitamin D on Dendritic Cells In Experimental Cerebral Malaria Mouse

Vitamin D (VD) is the steroid derivatives, it has the effect of anti-rickets, besides a certain immune function. The main immunomodulatory effects and mechanisms of VD on experimental cerebral malaria mouse were studied. The results showed that, as compared with control group, the C57BL/6 mice that administered VD prior to P. berghei ANKA (P.bANKA) infection, could prevent from cerebral malaria and the survival time was significantly prolonged. The percentages of myeloid dendritic cells (CD11c⁺CD11b⁺ cells) and plasmacytoid dendritic cells (CD11c⁺B220⁺ cells) in spleen were significantly reduced after pretreatment of VD. While the percentages of CD11c⁺MHCII⁺ cells, CD11c⁺TLR4⁺ cells and CD11c⁺TLR9⁺ cells in spleen were also decreased obviously. Thereby suggested that preventively oral VD could inhibit the number and function of dendritic cells, and had a preventive effect for cerebral malaria mice in C57BL/6 infected P.bANKA. This provided a theoretical basis for the research and development of new anti-malarial medicines.