| 门多萨假单胞菌NK-01合成中长链聚羟基脂肪酸酯的发酵条件优化 |
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| 引用本文： | 段静静,郭文斌,张弛,王媛媛,宫婷,宋存江. 门多萨假单胞菌NK-01合成中长链聚羟基脂肪酸酯的发酵条件优化[J]. 微生物学杂志, 2015, 0(4): 40-45 |
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| 作者姓名： | 段静静 郭文斌 张弛 王媛媛 宫婷 宋存江 |
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| 作者单位： | 1.厦门大学 环境与生态学院， 福建 厦门 361102；2.南开大学 生命科学学院，天津 300071;南开大学 生命科学学院，天津 300071;南开大学 生命科学学院，天津 300071;南开大学 生命科学学院，天津 300071;南开大学 生命科学学院，天津 300071;南开大学 生命科学学院，天津 300071 |
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| 基金项目： | 国家自然科学基金项目（31070039） |
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| 摘 要： | 为了提高PHAMCL在门多萨假单胞菌NK-01中的积累，采用单因素实验和正交实验确立了发酵生产PHAMCL的最佳条件，即以PHA产量为指标的最佳发酵条件为15 g/L葡萄糖浓度、C/N=50、发酵时间48 h，该条件下获得产量0.8 g/L以上的PHA；以PHA占菌体干重百分含量为指标的最佳发酵条件为10 g/L葡萄糖浓度、C/N=60、发酵时间48 h，该条件下获得占菌体干重50%以上的PHA。该研究将为门多萨假单胞菌NK-01用于PHAMCL的规模化生产提供理论依据。
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| 关 键 词： | 门多萨假单胞菌；聚羟基脂肪酸酯；正交实验 |
Optimization of Fermentation Conditions for Medium Chain Length Polyhydroxylalkanoate Synthesis by Pseudomonas mendocina NK-01 |
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| DUAN Jing-jing,GUO Wen-bin,ZHANG Chi,WANG Yuan-yuan,GONG Ting and SONG Cun-jiang. Optimization of Fermentation Conditions for Medium Chain Length Polyhydroxylalkanoate Synthesis by Pseudomonas mendocina NK-01[J]. Journal of Microbiology, 2015, 0(4): 40-45 |
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| Authors: | DUAN Jing-jing GUO Wen-bin ZHANG Chi WANG Yuan-yuan GONG Ting SONG Cun-jiang |
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| Affiliation: | 1. Coll. of Environ''t & Ecol., Xiamen Uni., Xiamen 361102; 2. Coll. of life Sci., Nankai Uni., Tianjin 300071;Coll. of life Sci., Nankai Uni., Tianjin 300071;Coll. of life Sci., Nankai Uni., Tianjin 300071;Coll. of life Sci., Nankai Uni., Tianjin 300071;Coll. of life Sci., Nankai Uni., Tianjin 300071;Coll. of life Sci., Nankai Uni., Tianjin 300071 |
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| Abstract: | In order to improve the accumulation of PHAMCL in vivo, single factor and orthogonal experiments were adopted to study the optimization fermentation conditions for Pseudomonas mendocina NK-01. When PHAMCL output was taken as an index, under optimized fermentation conditions at 15 g/L glucose, with C/N ratio at 50 and fermented for 48 h, produced over 0.8/L of PHAMCL. When the index was turned to PHAMCL percentage content of dried cell weight (DCW), the best fermentation conditions were 10 g/L glucose, with C/N ratio at 60 and fermented for 48 h, the PHAMCL output was over 50% of DCW. This study will provide theoretic foundations for large scale production of PHAMCL by P. mendocina NK-01. |
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