Physical and Chemical Properties of an Oncornavirus Associated with a Murine Adrenal Carcinoma Cell Line |
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| Authors: | W. Neal Burnette Charles H. Riggin William M. Mitchell |
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| Affiliation: | Departments of Microbiology and Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee 37232 |
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| Abstract: | A type C oncornavirus has been isolated from a continuous cell line of murine adrenal carcinoma in culture. The particles have a buoyant density of 1.165 g/cm(3), exhibit typical type C morphology by electron microscopy, possess an RNA-dependent DNA polymerase, and have a high molecular weight RNA (6.1 x 10(6)) which can be denatured to a homogeneous lower molecular weight species (3.2 x 10(6)) when extracted from rapidly harvested "immature" virions. The virus is related antigenically to other mammalian oncornaviruses and exhibits a similar, although much more complex, sodium dodecyl sulfate gel electrophoretic profile of virion proteins when compared to the profiles of other type C RNA tumor viruses. |
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