| 双乙酰还原酶的分离纯化研究 |
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| 引用本文： | 张介驰,于德水,田洁萍,彭万霖,庹莉.双乙酰还原酶的分离纯化研究[J].生物技术,1999,9(3):12-14. |
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| 作者姓名： | 张介驰 于德水 田洁萍 彭万霖 庹莉 |
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| 作者单位： | 1. 黑龙江省科学院应用微生物研究所,哈尔滨,150010
2. 齐齐哈尔教育学院,齐齐哈尔,161000 |
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| 摘 要： | 比较了５种细菌、４种酵母菌和鸡肝中双乙酰还原酶含量，其中粪肠杆菌（Ｅｎｔｅｒｏ－ｃｏｃｃｕｓｆａｅｃａｌｉｓ）ＡＳ１．５９５中含量较高，为９．６ＩＵ／ｇ湿菌体。并对ＡＳ１．５９５菌酶和鸡肝酶进一步经ＳｅｐｈａｄｅｘＧ－１００和ＤＺＡＥ柱分离纯化，提纯倍数分别为４５、８９３，最后收率分别为１８．５％、５．１％。
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| 关 键 词： | 双乙酰还原酶，分离，纯化 |
| 修稿时间： | 1998-10-31 |
ISOLATION,PURIFICATION OF DIACETYL REDUCTASE |
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| Zhang Jiechi,Yu Deshui,Tian Jieping,Peng Wanlin,Tuo Li.ISOLATION,PURIFICATION OF DIACETYL REDUCTASE[J].Biotechnology,1999,9(3):12-14. |
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| Authors: | Zhang Jiechi Yu Deshui Tian Jieping Peng Wanlin Tuo Li |
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| Abstract: | xperiments compared the
diacetyl reductase(DR)content of chick liver, bacter(five strains) and yeast (four strains), and DR
content of Enterococcus faecalis AS1.595 was highest(nearly 9.6 IU/g wet bacter). With sephadex
G_100 and DEAE cellulose 52, the DRs from AS1.595 and chick liver and were purified 45 folds
and 893 folds, and the final yields were 18.5%and 5.1% separately. |
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| Keywords: | diacetyl reductase isolation purification |
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