Determination of kynurenine in serum by high-performance liquid chromatography after enzymatic conversion to 3-hydroxykynurenine |
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| Authors: | J Naito I Ishiguro T Murazumi M Morimoto |
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| Affiliation: | 1. Department of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, Taiwan;2. Laboratory of Auditory Neurophysiology, Department of Neurosciences, Katholieke Universiteit Leuven, Leuven, Belgium;3. Department of Otolaryngology, Cheng Hsin General Hospital, Taipei, Taiwan;4. Faculty of Medicine, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan;6. Integrated Brain Research Unit, Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan;7. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan;8. Department of Speech Language Pathology and Audiology, College of Health Technology, National Taipei University of Nursing and Health Sciences, Taipei, Taiwan |
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| Abstract: | A method for the determination of the physiological level of kynurenine in human serum based upon conversion of kynurenine to 3-hydroxykynurenine by enzymatic reaction with the mitochondrial fraction and NADPH and analysis by reversed-phase high-performance liquid chromatography with electrochemical detection is described. Tryptophan gave no interference. For one analysis, 0.2 ml of serum was sufficient, compared with the large volume (5.0 ml) required for other methods. |
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