In vitro and ex vitro evaluation of long-term micropropagated turmeric as analyzed through cytophotometry,phytoconstituents, biochemical and molecular markers |
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Authors: | Sanghamitra Nayak Tanzeer Kaur Sujata Mohanty Goutam Ghosh Rakesh Choudhury Laxmikanta Acharya Enketeswara Subudhi |
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Institution: | (1) Centre of Biotechnology, Siksha O Anusandhan University, Khandagiri, Bhubaneswar, 751003, India;(2) School of Pharmaceutical Sciences, Siksha O Anusandhan University, Khandagiri, Bhubaneswar, 751003, India;(3) Jaypee University of Information Technology, Waknaghat, Solan, Simla, Himachal Pradesh, India |
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Abstract: | Turmeric (Curcuma longa L.), a high valued medicinal plant, was micropropagated through induction of multiple shoots using latent axillary buds of
rhizome. Cytophotometric and random amplified polymorphic DNA (RAPD) as well as inter simple sequence repeats (ISSR) analysis
were used to periodically monitor the genetic stability of micropropagated clones of Curcuma longa conserved in vitro up to 7 years at every 6 months interval. A total of eighteen RAPD and eight ISSR primers gave 45,537
distinct and reproducible bands, monomorphic across all 353 plants analyzed. Micropropagated turmeric after being conserved
for 7 years in vitro was transplanted into soil in field. Drug yielding potential of tissue culture derived plants was evaluated
in field through estimation of phytoconstituents like curcumin and essential oil contents. The result of 2 years of field
trial showed that micropropagated turmeric retained stability in all the characteristics examined when compared with the field
performance of conventionally propagated plants. Thus long term conservation of an elite genotype of turmeric with epigenetic
and genetic stability is significant for stable supply of drug i.e., curcumin and essential oil to the market. |
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