Production of saponions and polysaccharide in the presence of lactoalbumin hydrolysate in <Emphasis Type="Italic">Panax quinquefolium</Emphasis> L. cells cultures |
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Authors: | Juan Wang Wenyuan Gao Jian Zhang Tao Huang Tiantian Wen Liming Zhang Luqi Huang |
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Institution: | (1) School of Pharmaceutical Science and Technology, Tianjin University, 300072 Tianjin, China;(2) Tianjin University of Traditional Chinese Medicine, 300193 Tianjin, China;(3) Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, 300457 Tianjin, China;(4) Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 100700 Beijing, China; |
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Abstract: | In this article, ginsenosides and polysaccharide contents in suspension cells and native roots of Panax quinquefolium L. were studied. In order to enhance the contents of ginsenosides and polysaccharide in P. quinquefolium suspension cells, we tested the effects of lactoalbumin hydrolysate on the growth of P. quinquefolium suspension cell, synthesis of ginsenosides and polysaccharide in flask and bioreactor. In flask culture, cells growth ratio
was significantly enhanced by the addition of lower concentration of lactoalbumin hydrolysate. Addition of 100 mg L−1 lactoalbumin hydrolysate significantly enhanced the contents of total saponins (5.44 mg g−1 DW) and the contents were 3.89-fold over the control group. Addition of lactoalbumin hydrolysate significantly promoted the
accumulation of polysaccharide, except 200 mg L−1 lactoalbumin hydrolysate. The highest total saponins yield (36.72 mg L−1 DW) and polysaccharide yield (0.83 g L−1 DW) were obtained at 100 mg L−1 lactoalbumin hydrolysate. In a 5-L stirred tank bioreactor, the highest contents of total saponins and TRb group ginsenosides
were achieved on day 26, while the effect of lactoalbumin hydrolysate on the contents of TRg group ginsenosides were insignificant.
This result suggests that lactoalbumin hydrolysate might have triggered the enzyme activities for the synthesis of TRb group
ginsenosides. Overall, the highest total saponins yield (31.37 mg L−1 DW) and polysaccharide yield (1.618 g L−1 DW) were obtained on day 26 and day 24 respectively and the polysaccharide yield was 1.95-fold higher than the shake flask
culture (0.83 g L−1 DW). These results provided theoretical reference for two-stage culture in suspension cells of P. quinquefolium in bioreactor. |
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