Effects of combinational treatment with ethephon and indole-3-butyric acid on adventitious rooting of <Emphasis Type="Italic">Pinus thunbergii</Emphasis> cuttings |
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Authors: | Yasuhiro Mori Fumihiko Miyahara Yuji Tsutsumi Ryuichiro Kondo |
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Institution: | (1) Fukuoka Prefecture Forest Research and Extension Center, 1438-2, Toyoda Yamamoto-machi, Kurume, Fukuoka 839-0827, Japan;(2) Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan; |
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Abstract: | Adventitious rooting is essential for cutting propagation of pine wilt-resistant Pinus thunbergii. To examine a variety of adventitious rooting potentials among donor plants, cuttings were taken from 31 seedlings within
a half-sib family. Rooting abilities of cuttings from each seedling ranged from 0 to 100%. When 11 ortets and 11 ramets (clonally
propagated from each ortet) were used as donor plants, there was a positive correlation between rooting abilities of cuttings
from ortets and ramets, suggesting that adventitious rooting is dependent on genetic factors in the donor plants. To promote
adventitious rooting of cuttings by hormonal treatment, we examined the effect of soaking time in Oxyberon (19.7 mM indole-3-butyric
acid (IBA) solution) on rooting. Ten minutes was the best soaking time for rooted cuttings to produce more adventitious roots
without impairing normal growth. When cuttings were soaked in Ethrel diluent (69.2 μM ethephon) for 24 h before soaking in
Oxyberon for 10 min, a significantly higher rooting ability was observed than those soaked in Oxyberon alone. Ethrel on its
own barely affected rooting ability. The positive effect of the combinational treatment was confirmed in a similar experiment
using authentic ethephon and IBA instead of Ethrel and Oxyberon. When cuttings were soaked in a mixture of ethephon and silver
thiosulfate (STS), an ethylene action inhibitor, before IBA-soaking, the effect was partially diminished compared with combinational
treatment without STS. These findings suggest that ethylene action caused by ethephon treatment promotes IBA-mediated adventitious
rooting of P. thunbergii cuttings. |
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