Regulation of exogenous spermidine on the reactive oxygen species level and polyamine metabolism in <Emphasis Type="Italic">Alternanthera philoxeroides</Emphasis> (Mart.) Griseb under copper stress |
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Authors: | Xiaoying Xu Guoxin Shi Chunxia Ding Ye Xu Juan Zhao Haiyan Yang Qiuhong Pan |
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Institution: | (1) Jiangsu Key Lab of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, 1 Wenyuan Road, 210046 Nanjing, Jiangsu, People’s Republic of China; |
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Abstract: | Effects of exogenous spermidine (Spd) on the reactive oxygen species level and polyamine metabolism against copper (Cu) stress
in Alternanthera philoxeroides (Mart.) Griseb leaves were investigated. Cu treatment induced a marked accumulation of Cu and enhanced contents of malondialdehyde
(MDA), hydrogen peroxide (H2O2) and the generation rate of O2
·−. It also significantly increased putrescine (Put) levels but lowered spermidine (Spd) and spermine (Spm) levels. The activities
of arginine decarboxylase (ADC), ornithine decarboxylase (ODC) and polyamine oxidase (PAO) were all elevated with the increase
of Cu concentration. However, application of exogenous Spd effectively decreased H2O2 content and the generation rate of O2
·−, prevented Cu-induced lipid peroxidation and reduced Cu accumulation. Moreover, it declined level of endogenous Put and increased
levels of Spd and Spm. Activities of ADC, ODC and PAO were all inhibited by exogenous Spd. The results indicated that application
of exogenous Spd could enhance the tolerance of A. philoxeroides to Cu stress by reducing the reactive oxygen level and balancing polyamine metabolism. |
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