重复DNA顺序pRRD9在稻属中的拷贝数测定

本文应用狭缝印渍杂交方法,把水稻基因组总DNA和含水稻中度重复顺序片段的质粒(pRRD9)DNA分别转移到尼龙膜上形成狭缝印渍、然后用³²P标记的 pRRD9插入片段进行杂交、根据各狭缝印渍的放射性强度,测定水稻(Oryza)一些栽培种和野生种基因组中重复DNA顺序的拷贝数,并就拷贝数与水稻进化关系及基因组型的联系进行讨论.

DETERMINATION OF COPY NUMBER OF REPETITIVE DNA SEQUENCE pRRD9 IN GENUS ORYZA

Copy number of cloned repetitive DNA sequence pRRD9 has been determined within the genus Oryza, including 15 cultivated rice (7 belonging to Oryza sativa L. Japonica, 8 belonging to Oryza sativa L. Indica) and 16 wild rice entries (belonging to AA, A~I A~I, A~cu A~cu, BBCC, CC, CCDD, EE and FF genome types respectively). This was done by using quantitative filter hybridization technique named'SLOT-BLOTTING'. The result shows that pRRD9 copy number is markedly variable between cultivated and wild rice entr...