培养基上生长的黄瓜去根苗雌花高效诱导体系

报告了外源KT(激动素)和IAA(吲哚乙酸)对黄瓜去根苗雌花诱导的增效作用,以及外植体苗龄、下胚轴长度、培养基中N素和NH4 -N水平对成花的影响,据此建立了有效的雌花诱导体系。7d龄带1/2下胚轴的去根苗接种在MS培养基中,对KT3.0mg/L添加IAA0.01mg/L时的雌花成花率达28%,比单用KT3.0mg/L、IAA0.01mg/L的分别高12%、26%,而对照组未见雌花,说明KT和IAA对雌花诱导的增效作用显著。实验表明,N80mmol/L(NH4 -N/TN37.5%)、保留1/4下胚轴和6d苗龄时的黄瓜去根苗雌花诱导率最高。

A Highly Efficient System for Induction of Female Flowers in Derooted Seedlings of Cucumis sativus L. Grown on the Medium

This paper reported the synergistic effect of KT and IAA, and the effects of age of seedlings, length of hypocotyls, nitrogen contents and NH(+)(4)-N/TN ratio on the induction of female flowers in derooted seedlings of Cucumis sativus L. for the establishment of an efficient system for the induction of female flowers. When cultured on MS basal medium supplemented with KT 3.0 mg/L and IAA 0.01 mg/L, an induction rate of 28% was obtained for the 7-day-old derooted seedlings with 1/2 hypocotyl, 12% and 26% higher than when cultured with KT 3.0 mg/L alone and IAA 0.01 mg/L alone, respectively, while no female flowers was formed on the control medium, which indicates that KT and IAA have an evident synergistic effect on the induction of female flowers. Experiment was done with N concentration of 60-90 mmol/L or with NH(+)(4)-N/TN ratio of 12.5%-50% (total N kept at 80 mmol/L), with lengths of hypocotyls of 0-4/4, and with different ages of seedlings of 5-8-day-old, the highest induction rate of 40%, 48% and 57% for female flowers was achieved on medium of N 80 mmol/L (NH(+)(4)-N/TN 37.5%), from seedlings with 1/4 hypocotyl and from the treatment using 6-day-old seedlings respectively.