Transfer of DNA coding for cellulases from Cellulomonas species to Bacillus subtilis by protoplast fusion |
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| Authors: | D. V. Gokhale Eun Soo-Han V. R. Srinivasan Dileep N. Deobagkar |
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| Affiliation: | (1) Biochemistry Division, National Chemical Laboratory, 411 008 Pune, India;(2) Department of Microbiology, Lousiana State University, 70803 Baton Rouge, La, USA |
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| Abstract: | Summary Cellulose utilising hybrids between Cellulomonas spp. and Bacillus subtilis were isolated after PEG mediated protoplast fusion. These stable hybrids selected using genomic markers harboured DNA from both the parents. Higher CMCase and  -glucosidase activities were detected extracellularly in case of one of the hybrids unlike only CMCase in case of Cellulomonas.NCL Communication No. 3542 |
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