The Effects of Copper Sulfate on Liver Histology and Biochemical Parameters of Term Ross Broiler Chicks |
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Authors: | Emin Oguzhan Oguz Hayati Yuksel Yasar Enli A Cevik Tufan Gunfer Turgut |
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Institution: | (1) Department of Histology & Embryology, Faculty of Medicine, Pamukkale University, Gunes Binasi, Bursa Cad. No.125, 20070 Kinikli, Denizli, Turkey;(2) Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyon, Turkey;(3) Department of Biochemistry, Faculty of Medicine, Pamukkale University, Denizli, Turkey;(4) Department of Physiology, Faculty of Medicine, Pamukkale University, Denizli, Turkey |
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Abstract: | Copper is an essential trace element that is extremely toxic to organisms and organs at high doses. We have investigated the
histological and biochemical effects of a toxic dose of copper sulfate on the liver of term Ross broiler chicks. Fertilized
eggs were divided into three groups: experimental, injected with 50 mcg/0.1 ml copper sulfate in the air chambers on day 1;
sham, injected with 0.1 ml saline; and control, no injection. Term chicks were killed and their livers investigated histologically,
with hematoxylin–eosin-stained sections examined under light microscopy, and biochemically, for malondialdehyde and glutathione
levels. Histological examinations showed copper-treated samples with granular degeneration and necrosis of hepatocytes and
impairment to the cell lining of the remark cords. The samples had a congestive appearance, with blood in the vena centralis
and sinusoids, slight connective tissue increase, and lymphocyte infiltration. Control and sham group sections had normal
appearances. As oxidative damage parameters, in the copper-treated group, malondialdehyde levels were increased and glutathione
levels decreased. In the sham and control groups, there were no significant differences. At this toxic dose, copper sulfate
shows oxidative damage according to the histology of term chick liver that are confirmed biochemically by the changes in malondialdehyde
and glutathione levels. |
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