Total synthesis by modern chemical ligation methods and high resolution (1.1 A) X-ray structure of ribonuclease A |
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Authors: | Boerema David J Tereshko Valentina A Kent Stephen B H |
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Affiliation: | Department of Biochemistry and Molecular Biology, Gordon Center for Integrative Science, The University of Chicago, Chicago, IL 60637, USA. |
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Abstract: | The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution. |
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