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Isolation and characterization of an extracellular polysaccharide from Pseudomonas caryophylli CFR 1705 |
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| Authors: | S R Sudhamani R N Tharanathan M S Prasad |
| Institution: | a Department of Food Microbiology, Central Food Technological Research Institute, Mysore 570 013, India b Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore 570 013, India |
| Abstract: | Extracellular polysaccharides were isolated from Pseudomonas caryophylli CFR 1705 grown on lactose containing medium. The major fraction (no.1) obtained on DEAE-cellulose chromatography was composed of rhamnose, mannose and glucose in the ratio 1:3.26:4.97, respectively, and having a molecular weight of 1.1×106 Da. Methylation followed by GC-MS analysis revealed it to be a highly branched 1,4-linked hexosan with mannose and glucose as the branch-off residues at positions C-2 and C-6 of the main chain. Rhamnose was essentially found as non-reducing terminal residue. |
| Keywords: | Extracellular polysaccharide Pseudomonas caryophylli Purification Characterization Structure |
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