New expanded bed adsorbents for the recovery of DNA |
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| Authors: | Irini Theodossiou Morten A Olander Michael Søndergaard Owen RT Thomas |
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| Institution: | (1) Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark, Building 223, Søltofts Plads, DK-2800 Lyngby, Denmark;(2) UpFront Chromatography A/S, Lersø Parkallé 42, DK-2100 Copenhagen, Denmark |
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| Abstract: | A 20–40  m pellicular high density ( 3.7 g cm–3) expanded bed material has been designed for the capture of DNA and other large macromolecules. Anion exchangers fashioned out of these supports exhibited dramatically enhanced DNA binding capacities over commercial anion exchange adsorbents (6 mg ml–1, c.f. 50 g ml–1 at 10% breakthrough), due to a combination of small particle and  fuzzy surface architecture created through the coupling of polyethylene imine chains. |
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| Keywords: | anion exchange adsorbents DNA expanded bed chromatography gene therapy |
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