牛磺酸对急性低氧促培养的兔肺小动脉平滑肌细胞产生血栓素A2和前列环素的影响

本工作观察了牛磺酸（taurine）对分离培养家兔肺小动脉平滑肌细胞（PASMCs）在急性低氧条件下培养液中血栓素B2（TxB2）和6-酮-前列腺素F1α（6-keto-PGF1α）含量及其比值变化的影响,同时用环加氧酶抑制剂吲哚美辛干预,以探讨牛磺酸的作用途径。结果是：低氧使PASMCs培养液中TxB2和6-keto－PGF1α含量及其比值显著增加。常氧和低氧条件下,终浓度为2．5×10-5mol／L的牛磺酸显著降低TxB2的含量;显著提高6-keto-PGF1α的含量,终浓度为2．5×10－3mol／L的吲哚美辛显著降低TxB2和6-keto-PGF1α含量,牛磺酸和吲哚美辛共同作用,TxB2的含量进一步降低,6－keto-PGF1α含量比单纯用吲哚美辛增加明显。这些结果表明,牛磺酸可能通过抑制PASMCs内的TxA2合成酶的活性和增强前列环素（PGI2）合成酶的活性拮抗低氧增强PASMCs产生TxA2和PGI2,使TxA2与PGI2的比值显著降低而逆转低氧性肺血管收缩。

Effects of taurine enhancement of synthesis of TxA2 and PGI2 in cultured intra-pulmonary arteriolar smooth muscle cells under acute hypoxia

The results of the present study demonstrated that the concentrations of thromboxane B2 (TxB2a) and 6-keto-prostaglandin F1a. (6-keto-PGF1a), and their ratio were significantly increased in the medium of intra-pulmonary arteriolar smooth muscle cells (PASMCs) under acute, hypoxia. Under either normoxia or hypoxia the concentration of TxB2 was significantly decreased with addition of taurine, while that of 6-keto-PGF1a. was significantly increased. With the addition Of indomethacin, however, under similar conditions, the concentrations of both TxB2 and 6-keto-PGF1a. were decreased. Furthermore, the effect of indomathecin on TxA2 and 6-keto-PGF1a. concentrations could be revend by taurine.