Perturbed angular correlation gamma ray (PAC) spectroscopy of 111Cd carboxypeptidase A alpha. |
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| Authors: | R Bauer C Christensen J T Johansen J L Bethune B L Vallee |
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| Institution: | 1. The Niels Bohr Institute, University of Copenhagen, DK4000 Roskilde, Denmark;2. Department of Chemistry; Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK2100 Copenhagen, Denmark;3. Biophysics Research Laboratory, Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115 USA. |
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| Abstract: | The active center of Cd(II) carboxypeptidase A has been examined by means of perturbed angular correlation of gamma rays as a function of pH. This method simultaneously detects changes in coordination geometry, in the charge of groups within 5 Å of the metal nucleus and delineates multiple states of the active center. Carboxypeptidase A crystals exhibit one predominant state while several are present in solution. Interpretation of the experimental data in terms of Angular Overlap Theory suggests the species characteristic of the crystals has a distorted tetrahedral coordination geometry and is in equilibrium with a pentacoordinated species in solution. |
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