Effect of outer-membrane permeabilizers on the activity of antibiotics and plant extracts against<Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> |
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Authors: | A?Guha A?Choudhury B?G?Unni Email author" target="_blank">M?K?RoyEmail author |
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Institution: | (1) Biochemistyr Division, Regional Research Laboratory, 785 006 Jorhat, Assam, India |
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Abstract: | Several known outer membrane permeabilizers increased susceptibility of a highly resistant pathogenic strainPseudomonas aeruginosa to different antibiotics and plant extracts. Of all the chemicals tested, EDTA, sodium citrate and sodium hexametaphosphate
(HMP) were found to be potent permeabilizers as shown by enhanced lysis of the bacteria in the presence of lysozyme. In the
presence of EDTA and sodium citrate susceptibility of the strain to gentamicin and rifampicin increased markedly. The strain
was resistant to vancomycin but became susceptible when grown in the presence of increasing amounts of EDTA and sodium citrate.
Similar results were obtained for erythromycin when treated with sodium citrate. EDTA was found to be most potent permeabilizer
in enhancing the activity of the plant extracts. Though HMP was an effective permeabilizer it had a weak or no effect on the
activity of the antibiotics and plant extracts. |
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