Effects of some chemical factors on flagellation and swarming ofVibrio alginolyticus |
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Authors: | Willemina E de Boer Carol Golten W A Scheffers |
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Institution: | (1) Laboratory of Microbiology, Delft University of Technology, Delft, The Netherlands |
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Abstract: | Vibrio alginolyticus strains recently isolated from Dutch coastal seawater changed flagellar organization when cultivated in the presence of certain
chemical agents. On agar media with more than 4.0% (w/v) NaCl the number of lateral flagella per cell decreased with increasing
salt concentration. Both on agar media and in broth cultures with 6.0–9.0% (w/v) NaCl, cells with polar tufts of 2–4 sheathed
or unsheathed flagella were frequently found. Cells grown on agar media with 7.3–9.8% (w/v) Na2SO4 had drastically reduced numbers of lateral flagella, but lacked polar tufts.
EDTA suppressed growth, but did not affect flagellar arrangement. In the presence of 0.1–0.3% boric acid or 0.05–0.1% aluminium
hydroxide, cells in liquid media tended to produce lateral, in addition to the polar flagella normally observed in broth cultures.
Of a number of surface-active agents tested, Tween 80 and Na-taurocholate, even in high concentrations, did not affect flagellation.
Bile salts (0.1%) and Na-deoxycholate (0.05%) strongly reduced the number of both polar and lateral flagella. In agar cultures,
Na-lauryl sulphate (0.01–0.1%) inhibited the formation of lateral, but increased the incidence of polar flagella. Teepol (0.05–0.2%)
had a similar effect and also it had a deteriorating effect on the sheaths of the polar flagella.
Concomitant with the reduction in the number of lateral flagella, induced by these agents, swarming on agar media was inhibited. |
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