Monolayer culture of parenchymal rat hepatocytes on collagen-coated microcarriers. A hepatocyte system for short- and long-term metabolic studies |
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Authors: | L Agius C Battersby K G M M Alberti |
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Institution: | (1) Department of Clinical Biochemistry and Metabolic Medicine, The Medical School, University of Newcastle Upon Tyne, Newcastle Upon Tyne, NE2 4HH, England |
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Abstract: | Summary A method is described for the attachment to and monolayer culture of adult rat hepatocytes on collagen-coated or fibronectin-coated
microbeads or both in a chemically defined serum-free medium. Protein synthesis measured by the incorporation of 3H]leucine into protein was four-fold higher in the hepatocyte microcarrier cultures than in isolated hepatocyte suspensions.
The hepatocyte microcarrier cultures showed acute responsiveness to insulin of fatty acid synthesis, glucose incorporation
into glycogen, and decarboxylation of 1-14C]pyruvate. Microcarrier-cultured hepatocytes have the combined advantages of monolayer culture and suspension systems. They
are a potential tool for the study of long-term as well as acute effects of hormones.
This work was supported by the British Diabetic Association. |
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Keywords: | hepatocytes microcarriers cultures suspensions metabolic studies |
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