| 环介导恒温扩增对莱姆病病原伯氏疏螺旋体的分型鉴定 |
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| 引用本文： | 杨吉飞,关贵全,牛庆丽,李有全,刘军龙,马米玲,任巧云,刘爱红,王子坚,杨朝霞,罗建勋,殷宏.环介导恒温扩增对莱姆病病原伯氏疏螺旋体的分型鉴定[J].微生物学通报,2010,37(6):0866-0871. |
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| 作者姓名： | 杨吉飞 关贵全 牛庆丽 李有全 刘军龙 马米玲 任巧云 刘爱红 王子坚 杨朝霞 罗建勋 殷宏 |
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| 作者单位： | 中国农业科学院兰州兽医研究所,家畜疫病病原生物学国家重点实验室,甘肃省动物寄生虫病重点实验室,甘肃兰州,730046 |
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| 基金项目： | 国家支撑计划项目(No. 2007BAD40B02); 国家自然资源平台项目(No. 2005DKA21100); 国家自然科学基金项目(No. 30571397); 国家863计划项目(No. 2006AA10A207); 科技部欧盟项目专项经费项目(No. 245145); 中国农业科学院杰出人才基金、欧盟EPI-ZONE、ICTTD3 (INCO No. 510561); SSA-INCOME项目(No. 211757) |
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| 摘 要： | 使用环介导恒温扩增技术,基于莱姆病病原伯氏疏螺旋体的外膜蛋白A(OspA)基因,针对伯氏疏螺旋体不同的基因型设计特异性引物,对国内主要的莱姆病病原伯氏疏螺旋体的3个基因型进行分型鉴定。研究结果表明,设计的引物具有良好的特异性,可以对狭义伯氏疏螺旋体(Borrelia burgdorferi sensu strict)、嘎氏疏螺旋体(B.afzelii)和伽氏疏螺旋体(B.garinii)进行分型鉴定。伯氏疏螺旋体的分型鉴定可以对不同临床症状莱姆病患者的治疗和莱姆病的控制提供一定的依据。
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| 关 键 词： | 环介导的恒温扩增 伯氏疏螺旋体 分型鉴定 |
A Genotype Loop-mediated Isothermal Amplification (LAMP) for Borrelia Burgdorferi of Lyme Disease |
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| YANG Ji-Fei,GUAN Gui-Quan,NIU Qing-Li,LI You-Quan,LIU Jun-Long,MA Mi-Ling,REN Qiao-Yun,LIU Ai-Hong,WANG Zi-Jian,YANG Zhao-Xi,LUO Jian-Xun and YIN Hong.A Genotype Loop-mediated Isothermal Amplification (LAMP) for Borrelia Burgdorferi of Lyme Disease[J].Microbiology,2010,37(6):0866-0871. |
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| Authors: | YANG Ji-Fei GUAN Gui-Quan NIU Qing-Li LI You-Quan LIU Jun-Long MA Mi-Ling REN Qiao-Yun LIU Ai-Hong WANG Zi-Jian YANG Zhao-Xi LUO Jian-Xun and YIN Hong |
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| Institution: | Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China;Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lan-zhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China |
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| Abstract: | Borrelia Burgdorferi is the causative pathogens for Lyme disease. A loop-mediated isothermal amplification (LAMP) rapid assay was developed for genotyping Borrelia borgdorferi based on the outer surface protein A (OspA) gene. All three different genotypic isolates including B. burgdorferi sensu stricto, B. afzelii and B. garinii, distributed mainly in China were detected and genotyped successfully using the LAMP method. The genotyping of Borrelia burgdorferi would provide evidence for treatment of patients with different clinical symptoms and for control of Lyme disease. |
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| Keywords: | Loop-mediated isothermal amplification Borrelia burgdorferi Genotyping |
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