Effect of lectin from the ascidian on the growth and the adhesion of HeLa cells |
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Authors: | Odintsova Nelly A Belogortseva Natali I Khomenko Alexander V Chikalovets Irina V Luk'yanov Pavel A |
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Institution: | (1) Laboratory of Cell Biophysics, Institute of Marine Biology, Far East Branch of RAS, Vladivostok, 69004, Russia;(2) Laboratory of Chemistry of Non infectious Immunity, Institute of Bioorganic Chemistry, Far East Branch of RAS, Vladivostock, 690022, Russia |
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Abstract: | Cell adhesion molecules, some of which are lectins, play a key role in the control of normal and pathological processes of various living organisms. We found herein that N acetyl D glucosamine specific lectin, isolated from the ascidian Didemnum ternatanum (DTL), alters the growth properties of HeLa tumor cells depending on the anchorage. DTL was shown to increase the proliferation of HeLa cells grown in soft agar greatly (in anchorage independent fashion). In contrast, DTL inhibits the proliferative activity of HeLa cells grown on solid substrate and acts as inductor of differentiation, slowing cell growth, increasing the cell attachment and spreading. Scanning electron microscopic data have demonstrated that DTL treatment resulted in pronounced changes of the shape and surface of HeLa cells. Changes of cellular morphology correlated with essential redistribution of actin microfilaments. |
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Keywords: | ascidian lectin adhesion growth factor HeLa cells tumor cells |
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