RFLP- and physical mapping of resistance gene homologues in rice (O. sativa) and Barley (H. vulgare) |
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| Authors: | D Leister J Kurth D A Laurie M Yano T Sasaki A Graner P Schulze-Lefert |
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| Institution: | (1) The Sainsbury Laboratory, John Innes Centre, Colney Lane, NR4 7UH Norwich, UK, GB;(2) The John Innes Centre, Colney Lane, NR4 7UH Norwich, UK, GB;(3) Rice Genome Research Program/STAFF Institute, 446-1, Ippaizuka, Tsukuba, Ibaraki 305, Japan, JP;(4) Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Corrensstr. 3, D-06466 Germany, DE |
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| Abstract: | The deduced peptide sequences of 25 gene fragments of NBS-LRR resistance (R) gene homologues from rice and barley and of characterized R genes were compared, revealing a string of six conserved motifs. Mapping of the R-gene candidates in rice showed linkage to genes conferring race-specific resistance to rice blast (Pi-k, Pi-f and Pi-1) and bacterial blight disease (Xa-1, Xa-3 and Xa-4), in barley to powdery mildew (Mla) and the rust fungus (Rpg1). In rice four mixed clusters were detected, each harboring at least two highly dissimilar NBS-LRR genes. A YAC-contig was
established for one of these mixed clusters. YAC fragmentation experiments revealed the presence of at least five NBS-LRR
genes within 200 kb in head-to-tail orientation.
Received: 24 July 1998 / Accepted: 14 August 1998 |
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| Keywords: | Resistance gene RFLP YAC contig Fragmentation Gene cluster |
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