Protein deficiency and age related alterations in rat peritoneal macrophage lipids |
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Authors: | J P Machaiah U K Vakil |
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Institution: | (1) Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Trombay, 400 085 Bombay, India |
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Abstract: | The effects of dietary protein restriction and age on the thioglycollate elicited peritoneal macrophage lipid constituents
were studied. Impact of subtle changes in lipid components on macrophage functions have been assessed. Lipid profiles of macrophages
recovered from rats fed 20 and 4% protein diets and stock diet fed rats (0 and 3 wk) were comparable qualitatively. Quantitative
analysis however revealed significant decrease in phospholipids (30–40%) and consequent elevation of cholesterol/phospholipid
molar ratios in the protein depleted and young rats (0 wk), compared to the protein fed groups. The protein deficient and
the young rats also exhibited accumulation of certain neutral lipids and reduction in triglycerides. Analysis of fatty acid
methyl esters of macrophage phospholipids revealed the predominance of long chain polyunsaturated fatty acids even when oleic
(C18:1) and linoleic (C18:2) formed the bulk of unsaturated fatty acids in the diet. However, the long chain poly unsaturated fatty acid content, particularly
the docosahexaenoic acid (C22:6n-3) was greatly reduced in the protein depleted and 0 wk rats. Observed changes in the long chain polyunsaturated fatty acids
of macrophage phospholipids may be of physiological significance as they modulate the immunological functions of the cell. |
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Keywords: | Protein deficiency peritoneal macrophages lipids cholesterol/phospholipid ratio fatty acid composition |
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