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Argiopinin-binding proteins from the membrane of the bull cerebral cortex |
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| Authors: | T M Volkova N A Avetisian T G Galkina A B Kudelin E M Makhmudova M M Solov'ev B A Tashmukhamedov E V Grishin |
| Abstract: | The 40 kDa argiopinin-binding glycoprotein has been isolated from the solubilised preparations of bovine cerebrum membranes by means of two-step biospecific chromatography on affinity sorbents with immobilized glutamate and argiopinins. This receptor component displays a specific L-3H]glutamate binding with Kd = 0.18 +/- +/- 0.019 mumole and Bmax = 43 +/- 4.5 nmole/mg. Amino acid analysis reveals it to be a member of integral membrane proteins. |
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