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Creation of a HEK293 cell line stably expressing the proteasome subunit PSMD14 fused with fluorescent protein EGFP and HTBT tag |
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| Authors: | V A Kulichkova Yu Ya Zaykova Yu B Ermolaeva E V Vinogradova L Huang A N Tomilin N A Barlev A S Tsimokha |
| Institution: | 1. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia 2. St. Petersburg State University, St. Petersburg, Russia 3. University of California, Irvine, USA 4. St. Petersburg State Technological Institute, St. Petersburg, Russia 5. University of Leicester, Leicester, UK |
| Abstract: | In the present work, a stable cell line based on human embryonic kidney HEK293 cells, which expresses the proteasome subunit PSMD14 fused to the fluorescent protein EGFP and HTBH tag, has been selected. This protein, PSMD14-EGFP-HTBH, was shown to be completely incorporated into proteasomes, and such complexes have a chymotrypsin-like peptidase activity that is fundamental for proteasomes. The constructed and selected cell line can be used for further fluorescent studies of localization of proteasomes in the cell. |
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