| CO2对转基因鱼腥藻生长的影响 |
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| 引用本文： | 张晨,刘志伟,郭勇. CO2对转基因鱼腥藻生长的影响[J]. 生物技术, 2003, 13(4): 27-29 |
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| 作者姓名： | 张晨 刘志伟 郭勇 |
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| 作者单位： | 1. 嘉应学院生物系,广东,梅州,514015
2. 华南理工大学食品与生物工程学院,广东,广州,510640 |
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| 基金项目： | 广东省自然科学基金资助项目("转基因海藻的构建及其培养技术研究",980541) |
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| 摘 要： | 为了进一步探索转基因鱼腥藻高密度培养的方法,在小型气升式反应器中研究了CO2对转基因鱼腥藻7120培养的影响。结果发现转基因鱼腥藻培养过程中通入5％ CO2能促进藻细胞生长,12d生物量提高7．44％,由于光照限制,不能大幅提高15d收获生物量,但生长周期能缩短近20％;而高浓度(10％)的CO2抑制转基因鱼腥藻的生长。CO2是通过调节pH值和影响碳源利用来影响藻细胞生长的,合适浓度的CO2有利于转基因鱼腥藻的培养。
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| 关 键 词： | CO2 转基因鱼腥藻 生长 高密度培养 α型重组TNF衍生物 |
| 文章编号： | 1004-311X(2003)04-0027-03 |
| 修稿时间： | 2003-02-18 |
Effect of CO2 on the Growth of Recombinant Anabaena sp.PCC7120 |
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| ZHANG Chen ,LIU Zhi-wei ,GUO Yong. Effect of CO2 on the Growth of Recombinant Anabaena sp.PCC7120[J]. Biotechnology, 2003, 13(4): 27-29 |
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| Authors: | ZHANG Chen LIU Zhi-wei GUO Yong |
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| Affiliation: | ZHANG Chen 1,LIU Zhi-wei 1,GUO Yong 2 |
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| Abstract: | Effect of CO 2 was studied on the culture of recombinant Anabaena sp. PCC7120 in air-lift bioreactor.The results showed that air contained 5% CO 2 can promote the cell growth,and 12d biomass was increased 7.44%. Because light is a limited factor,the 15d harvested biomass can't be increased remarkably,but the culture period can be shortened about 20%.The growth of Recombinant Anabaena sp .PCC7120 will be inhibited by high concentration of CO 2.CO 2 influences the growth of algae cell by adjusting pH and the utilization of carbon source in culture media,and suitable concentration of CO 2 was benefit for the culture of recombinant Anabaena sp . PCC7120. |
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| Keywords: | Recombinant Anabaena sp . PCC7120 CO 2 growth |
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