Binding of 3-carbethoxipsoralen to human serum albumin and human serum: influence of free fatty acids |
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| Authors: | G Font J Ma?es H Martre P Prognon G Mahuzier |
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| Institution: | Laboratoire de Chimie Analytique II, Faculté de Pharmacie, Université de Paris-Sud, Chatenay-Malabry, France. |
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| Abstract: | Characteristics of the binding of 3-carbethoxipsoralen (3CPS) to human serum albumin (HSA) and serum proteins have been studied. An electrophoretic study showed that the predominant binding protein fraction was albumin, with small binding to globulins. Binding to HSA, studied by equilibrium dialysis, is 75% and characterized by a small saturable number of binding sites (N = 0.27) with a moderate affinity constant (K = 8 X 10(4) M-1). Free fatty acids were shown to decrease 3CPS binding to HSA by a non competitive process. |
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