Electron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin |
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Authors: | J I Rowntree A Watts |
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Institution: | Department of Biochemistry, University of Oxford, England. |
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Abstract: | Bovine rhodopsin was isolated in the unbleached form as a retinal disc membrane suspension and spin-labelled with 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl. Both conventional and saturation transfer electron spin resonance methods were used to investigate the sensitivity of the spin-label to conformational changes of rhodopsin induced by both transient and long-term exposure to light. The results indicate that the ESR methods do display sensitivity to such changes. An exponential decay curve with a time constant of 10 s was obtained by following the height of a single peak in the saturation transfer electron spin resonance spectrum in response to a single light flash. |
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