| 中国4个民族mtDNA D环多态性研究 |
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| 引用本文： | 陈伟,陈峰,薛亚丽,杨焕杰,傅松滨,张贵寅,李璞. 中国4个民族mtDNA D环多态性研究[J]. 人类学学报, 2000, 19(2): 127-131 |
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| 作者姓名： | 陈伟 陈峰 薛亚丽 杨焕杰 傅松滨 张贵寅 李璞 |
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| 作者单位： | 哈尔滨医科大学医学遗传学研究室,哈尔滨,150086 |
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| 基金项目： | 本课题为国家自然科学基金重大项目资助，课题号：39392900 |
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| 摘 要： | 本文利用聚合酶链反应限制性片长度多态性（ＰＣＲ－ＲＦＬＰ）方法，检测了３６名汉族，３０名达斡尔族，３２名鄂伦春族，３０名鄂温克族随机选择正常个体ｍｔＤｎＡ Ｄ环４６５ｂｐＤＮＡ片多性并加以比较。结果表明，ｍｔＤＮＡ Ｄ环该片段的ＲＦＬＰ分析共产生２７种限制性类型，计算出４个民族的ｍｔＤＮＡ Ｄ环平均核苷酸歧异频率，并对４个民族的亲缘关系进行了聚类分析。
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| 关 键 词： | mtDNA D环 遗传多态性 PCR－RFLP 民族 亲缘关系 |
| 修稿时间： | 1999-04-09 |
STUDY ON THE mtDNA D LOOP POLYMORPHISM OF FOUR ETHNIC GROUPS IN CHINA |
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| Chen Wei,Chen Feng,Xue Yali,Yang Huanjie,Fu Songbin,Zhang Guiyin,Li Pu. STUDY ON THE mtDNA D LOOP POLYMORPHISM OF FOUR ETHNIC GROUPS IN CHINA[J]. Acta Anthropologica Sinica, 2000, 19(2): 127-131 |
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| Authors: | Chen Wei Chen Feng Xue Yali Yang Huanjie Fu Songbin Zhang Guiyin Li Pu |
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| Affiliation: | Chen Wei,Chen Feng,Xue Yali,Yan Huanjie,Fu Songbin,Zhang Guiyin;(Laboratory of Medical Genetics, Harbin Medical University, Harbin 150086) |
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| Abstract: | The PCR-RFLP techniques were used to study the mtDNA D loop polymorphism in Han (36 testees), Daur (30 testees), Oroqen (32 testees) and Ewenki (30 testees).All testees were randomly selected and healthy. The polymorphisms on 10 sites of a 465bplong DNA fragment in mtDNA D loop were detected and compared among ethnic groups.Altogether 27 mitotypes were found by PCR-RFLP.The mean nucleic acid substitute frequency on each site was Han 0.0291, Daur 0.0541, Oroqen 0.0477 and Ewenki 0.0563, respectively. |
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| Keywords: | mtDNA D loop Genetic polymorphism |
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